Comparative Evaluation of PCR and Imprint Smear Microscopy of Skin
نویسندگان
چکیده
27 Diagnosis of Post Kala-azar dermal leishmaniasis (PKDL), particularly the macular form is 28 difficult by microscopy. This study compared the positivity of nested polymerase chain reaction 29 (91.9%) with imprint smear microscopy (70.9%) in 62 PKDL samples. Nested PCR, showing 87.5% 30 positivity in macular lesions compared to 41.6% by imprint smear microscopy, is an efficient method 31 for early diagnosis of PKDL. 32 33 Running Title: PCR vs microscopy in PKDL diagnosis. 34
منابع مشابه
Comparative evaluation of PCR and imprint smear microscopy analyses of skin biopsy specimens in diagnosis of macular, papular, and mixed papulo-nodular lesions of post-kala-azar dermal leishmaniasis.
Diagnosis of post-kala-azar dermal leishmaniasis (PKDL), particularly the macular form, is difficult when based on microscopy. This study compared the results of nested PCR (91.9% positive samples) with imprint smear microscopy (70.9% positive samples) for 62 PKDL samples. We found that nested PCR, which indicated 87.5% positivity for the macular lesions, compared to 41.6% positivity by imprint...
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